Simple Spectrophotometric and Conductometric Methods for Determination of Gemifloxacin in Pure, Pharmaceutical Dosage Form and Human Urine

Article history: Received on: 17/09/2015 Revised on: 09/01/2016 Accepted on: 03/03/2016 Available online: 28/12/2016 Two Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation ofgemifloxacin (GEM) in pharmaceutical dosage forms and biological human urine. Method A: A spectrophotometric method is based on the oxidation reaction between phosphomolybdic acid (PMA) and GEM to form molybdenum blue (Mo +5 ). Beer , s law was obeyed in the concentration range of (5-27 μg/ml). The correlation coefficient ( 2) for the studied drug was found to be 0.9999. The molar absorptivity ( ), Sandells sensitivity, limit of detection (LOD), and limit of quantitation (LOQ)were also calculated. Method B: A conductometric method is based on formation of an ion associate with PMA. It involves direct titration with PMA in the range of 1-20 mg. The precipitate obtained by ion pairing GEM with PMA has been spectroscopically characterized using IR spectroscopy. The method was successively applied to pharmaceutical formulations containing GEM. The results obtained were favorably compared with those obtained using the reported method.